Journal:

Article Title: Replicate PCR Testing and Probit Analysis for Detection and Quantitation of Chlamydia pneumoniae in Clinical Specimens

doi: 10.1128/JCM.39.5.1796-1801.2001

Figure Lengend Snippet: Number of positives per 10 replicates versus the concentration of C. pneumoniae ATCC VR-1310 for non-nested PCR (A) and nested PCR (B) and a regression curve determined by probit regression analysis (SPSS).

Article Snippet: At a concentration of 1 IFU/ml, the non-nested PCR detected 5 of 10 versus 8 of 10 for the nested PCR; and at a concentration of 0.1 IFU/ml, the non-nested PCR detected 0 of 10 versus 1 of 10 for the nested PCR. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 1 caption a7 Number of positives per 10 replicates versus the concentration of C. pneumoniae ATCC VR-1310 for non-nested PCR (A) and nested PCR (B) and a regression curve determined by probit regression analysis (SPSS).

Techniques: Concentration Assay, Nested PCR

Journal:

Article Title: Replicate PCR Testing and Probit Analysis for Detection and Quantitation of Chlamydia pneumoniae in Clinical Specimens

doi: 10.1128/JCM.39.5.1796-1801.2001

Figure Lengend Snippet: Comparison of probit regression curves for a non-nested (●) and nested (▴) C. pneumoniae PCR (SPSS). The probit (predicted proportion of replicates positive) versus the C. pneumoniae ATCC VR-1310 concentration was obtained from 10 replicates of 16 dilutions (see the text).

Article Snippet: At a concentration of 1 IFU/ml, the non-nested PCR detected 5 of 10 versus 8 of 10 for the nested PCR; and at a concentration of 0.1 IFU/ml, the non-nested PCR detected 0 of 10 versus 1 of 10 for the nested PCR. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 1 caption a7 Number of positives per 10 replicates versus the concentration of C. pneumoniae ATCC VR-1310 for non-nested PCR (A) and nested PCR (B) and a regression curve determined by probit regression analysis (SPSS).

Techniques: Concentration Assay

Journal:

Article Title: Replicate PCR Testing and Probit Analysis for Detection and Quantitation of Chlamydia pneumoniae in Clinical Specimens

doi: 10.1128/JCM.39.5.1796-1801.2001

Figure Lengend Snippet: Predicted probability of PCR positive test versus the C. pneumoniae concentration for nested PCR by number of replicates. Replicates of 10, 5, 3, or 2 versus single PCR illustrated from left to right. Single PCR curves obtained from probit regression analysis of 10 replicates of 16 dilutions of C. pneumoniae ATCC VR-1310 (see text) are also shown. The remaining four curves were calculated in the spreadsheet program Microsoft Excel using the following formula: probability (at least 1 positive in n replicates) = 1 − (1 − probit)n.

Article Snippet: At a concentration of 1 IFU/ml, the non-nested PCR detected 5 of 10 versus 8 of 10 for the nested PCR; and at a concentration of 0.1 IFU/ml, the non-nested PCR detected 0 of 10 versus 1 of 10 for the nested PCR. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 1 caption a7 Number of positives per 10 replicates versus the concentration of C. pneumoniae ATCC VR-1310 for non-nested PCR (A) and nested PCR (B) and a regression curve determined by probit regression analysis (SPSS).

Techniques: Concentration Assay, Nested PCR